- anti Human, Mouse INTRACELL -

Reference Size Price Quantity Add to cart
INTRA 50 test
91,00 €

Product Description

  • FCM
  • Human, Mouse
  • NaN3 (Sodium azide)
  • IVD-CE
  • IntraCell is intended for fixation and permeabilization of cells in suspension for flow cytometry analysis
  • Store in the dark at 2-8 ºC
  • Link

Staining cells for internal antigens techniques have been devised for permeabilizing cells so that specific antibody can diffuse in and out and access internal antigens. Briefly, cells are fixed with formaldehyde to preserve their morphology, and saponin, a plant glycoside and mild nonionic detergent, is used to generate pores of approximately 8nm in the membrane which allow molecules of up to 200 kDa to pass through. Because of slow diffusion in and out of the cell, longer staining and washing times are required, cell and antibody preparations must be of high quality, and antibody must be at optimal titre to reduce nonspecific background. Other treatments can be used. For staining nucleic acid, fixation and permeabilization with alcohol and acetic acid reduces degradation of RNA and DNA. Cells may also be stained with antibody to a cell surface antigen for multicolour analysis. IntraCell is intended for fixation and permeabilization of cells in suspension for flow cytometry analysis. Immunological detection studies of intracellular antigens on structures such as cytoplasmic and/or nuclear enzymes, requires the permeabilization of the cell membrane in order to allow interaction of the antibody with its intended target.. In order to allow recognizable cellular integrity after permeabilization a fixation step involving cross-linking or denaturation is required. This is usually accomplished by aldehyde or alcohol fixation followed by detergent permeabilization of the cell membrane. Labeling of cell surface antigens with antibodies prior to the fixation step is possible thus allowing simultaneous surface antigens phenotyping with internal antigen expression in multiparameter fluorescent cytometric analysis. IntraCell is developed for use with all flow cytometers and reagents remains intact the cell surface marker expression and the cell properties of FSC and SSC.

References:

  • Mamede A.C. et al. Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma. 2016 Pathol. Oncol. Res.
  • Navarro R. et al. Role of nucleotide-binding oligomerization domain 1 (NOD1) in pericyte-mediated vascular inflammation. Feb. 2016 The Authors. Journal of Cellular and Molecular 
  • Fernández-Sánchez A. et al. DNA demethylation and histone H3K9 acetylation determine the active transcription of the NKG2D gene in human CD8+ T and NK cells. Epigenetics. 2013 Jan 1; 8(1): 66–78
  • Santoro A. et al. Relationship between CK19 expression, deregulation of normal keratinocyte differentiation pattern and high risk-human papilloma virus infection in oral and oropharyngeal squamous cell carcinoma. Infect Agent Cancer. 2015; 10: 46.
  • Villar M. et al. Identification and Characterization of Anaplasma phagocytophilum Proteins Involved in Infection of the   Tick Vector, Ixodes scapularis. PLoS One. 2015; 10(9): e0137237.
  • Ayllón N. et al. Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells. Infect Immun. 2013 Jul; 81(7): 2415–2425.
  • Naranjo V. et al. Reciprocal Regulation of NF-kB (Relish) and Subolesin in the Tick Vector, Ixodes scapularis. PLoS One. 2013; 8(6): e65915.
  • Mendes J. et al. L744,832 and Everolimus Induce Cytotoxic and Cytostatic Effects in Non-Hodgkin Lymphoma Cells. Pathol. Oncol. Res. DOI 10.1007/s12253-015-9998-4
  • Brito AF. Et al.  Hepatocellular Carcinoma and Chemotherapy: The Role of p53. Chemotherapy 2012;58:381–386.  DOI: 10.1159/000343656 
  • Casalta-Lopes J. et al. Efflux Pumps Modulation in Colorectal Adenocarcinoma Cell Lines: The Role of Nuclear Medicine. Journal of Cancer Therapy. Vol.2 No.3(2011), Article ID:6693,10 pages DOI:10.4236/jct.2011.23056
  • Brito AF et al. Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression. 2015 JOURNAL OF CLINICAL AND EXPERIMENTAL HEPATOLOGY
  • Mamede AC. Et al. Selective cytotoxicity and cell death induced by human amniotic membrane in hepatocellular carcinoma. 2015 Med Oncol

Tech Data Sheet

TDS_INTRA-50T

Material safety Data Sheet

MSDS_Intracell.pdf