FITC Annexin V Apoptosis Detection Kit with PI

Reference Size Price Quantity Add to cart
ANXVKF-100T 100 test
215,00 €

Product Description

  • Flow Cytometry
  • FITC
  • All mammalian
  • The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • IVD-CE
  • This kit is intended for RUO in the identification of apoptotic cells. For flow cytometric staining, the suggested use of this reagent is 5 µl per 1 - million cells in a 100 µl volume of Annexin V Binding Buffer
  • Store in the dark at 2-8 ºC
  • Link

Apoptosis is characterized by a variety of morphological features. One of the earliest indications of apoptosis is the translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane. Once exposed to the extracellular environment, binding sites on PS become available for Annexin V, a 35-36 kDa, Ca 2+-dependent, phospholipid binding protein with a high affinity for PS. The translocation of PS precedes other apoptotic processes such as loss of plasma membrane integrity, DNA fragmentation, and chromatin condensation. As such, Annexin V can be conjugated to biotin or to a fluorochrome , and used for the easy, flow cytometric identification of cells in the early stages of apoptosis.

Sanz. B et al. Magnetic hyperthermia enhances cell toxicity with respect to exogenous heating. 2016 Biomaterials.

Serra S. et al. Adenosine signaling mediates hypoxic responses in the chronic lymphocytic leukemia microenvironment. Blood Advances 2016

Hohenforst-Schmidt W. et al. COX-2 Inhibitors, a Potential Synergistic Effect with Antineoplastic Drugs in Lung Cancer. Oncomedicine 2017.

Roel M et al. Marine guanidine alkaloids crambescidins inhibit tumor growth and activate intrinsic apoptotic signaling inducing tumor regression in a colorectal carcinoma zebrafish xenograft model. 2016 Oncotarget.

Sanz B. et al. Magnetic hyperthermia enhances cell toxicity with respect to exogenous heating.

Fernández-Moreno M et al. Generating Rho-0 Cells Using Mesenchymal Stem Cell Lines. October 2016. PlosOne

Muela A. et al. Optimal Parameters for Hyperthermia Treatment Using Biomineralized Magnetite Nanoparticles: A Theoretical and Experimental Approach. 2016 The Journal of Physical Chemistry C.

Bigolin A. et al.  INVESTIGAÇÃO DO MECANISMO DE MORTE CELULAR INDUZIDO POR COMPOSTOS EM CÉLULAS DE LINHAGENS DE NEOPLASIAS HEMATOLÓGICAS. Florianópolis 2016.

Tricarico P.M et al. HIV Protease Inhibitors Apoptotic Effect in SH-SY5Y Neuronal Cell Line. 2016 Cell Physiol Biochem

Bas E. et al. Selected polyphenols potentiate the apoptotic efficacy of glycolytic inhibitors in human acute myeloid leukemia cell lines. Regulation by protein kinase activities. 2016 Cancer Cell International.

Al-achbili LM et. al ITH33/IQM9.21 provides neuroprotection in a novel ALS model based on TDP-43 and Na+/Ca2+ overload induced by VTD. 2016 Neuroscience Letter.

Díaz-Núñez, M et al. Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity. 2016 BMC Cancer.

Salome Pires et al. Ascorbic acid and colon cancer: An oxidative stimulus to cell death depending on cell profile. 2016 European Journal of Cell Biology.

Contreras M. et al. Vaccinomics Approach to Tick Vaccine Development. 2016. Volume 1404 of the series Methods in Molecular Biology pp 275-286.

Ferreiro S.F. et al. Subacute cardiotoxicity of yessotoxin: in vitro and in vivo studies. 22th April 2016. Chem. Res. Toxicol.

García Alía et al. Estudio del perfil oxidativo en células ciliadas del epitelio respiratorio nasal de pacientes con Síndrome de Discinesia Ciliar Primaria. 2016 Universidad Politécnica de Valencia.

Mendes F. et al. Single Shot Irradiation and Molecular Effects on a Diffuse Large B Cell Lymphoma Cell Line. 2016 Journal of Cancer Research and Treatment.

Marcuzzi A et al. Geranylgeraniol and Neurological Impairment: Involvement of Apoptosis and Mitochondrial Morphology. 2016 Int. J. Mol. Sci.

Voudoukis et al. Distinct features of circulating microparticles and their relationship with disease activity in infl ammatory bowel disease. 2016 Annals of Gastroenterology.

Fernández et al. Experimental Basis for the High Oral Toxicity of Dinophysistoxin 1: A Comparative Study of DSP. Toxins 2014

Herrero et al. Deregulation of DNA Double-Strand Break Repair in Multiple Myeloma: Implications for Genome Stability. PLOS ONE. 2015.

Llanos Valero et al. E1a promotes c-Myc-dependent replicative stress Implications in glioblastoma radiosensitization. Cell Cycle 13:1, 52–61; January 1, 2014.

Moreno-Ortega et al. CALHM1 and its polymorphism P86L differentially control Ca2+ homeostasis, mitogen-activated protein kinase signaling, and cell vulnerability upon exposure to amyloid β. Aging Cell (2015)

Misiewicz-Krzeminsk et al. Restoration of microRNA-214 expression reduces growth of myeloma cells through positive regulation of P53 and inhibition of DNA replication. Haematologica. 2013

Sánchez-Carrera et al. ∆Np73 is capable of inducing apoptosis by co-ordinately activating several BH3-only proteins. Biosci Rep. 2015.

García-Cano et al. Exploiting the potential of autophagy in cisplatin therapy: A new strategy to overcome resistance. Oncotarget. 2015

Guglielmelli et al. mTOR pathway activation in multiple myeloma cell lines and primary tumour cells: pomalidomide enhances cytoplasmic-nuclear shuttling of mTOR protein. Oncoscience. 2015.

Ayllon N et al. Systems Biology of Tissue-Specific Response to Anaplasma phagocytophilum Reveals Differentiated Apoptosis in the Tick Vector Ixodes scapularis. PLoS Genet. 2015.

Rubattu S et al. The C2238/αANP Variant Is a Negative Modulator of Both Viability and Function of Coronary Artery Smooth Muscle Cells. PLoS One. 2014.

Paino T. et al. Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138++ and CD138low Subpopulations in Multiple Myeloma Cell Lines. PLoS One. 2014.

Rubiolo J.A. et al. Mechanism of cytotoxic action of crambescidin-816 on human liver-derived tumour cells. Br J Pharmacol. 2014

Dicarlo E. et al. The antitumor potential of Interleukin-27 in prostate cancer. Oncotarget. 2014.

Rubiolo J. A. et al. Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae. Mar Drugs. 2013.

López-Huertas M.R. et al. The Presence of HIV-1 Tat Protein Second Exon Delays Fas Protein-mediated Apoptosis in CD4+ T Lymphocytes. J Biol Chem. 2013.

François S. et al. Necdin Enhances Myoblasts Survival by Facilitating the Degradation of the Mediator of Apoptosis CCAR1/CARP1. PLoS One. 2012.

Peron M. et al. Effect of inhibition of the Ubiquitin-Proteasome System and Hsp90 on growth and survival of Rhabdomyosarcoma cells in vitro. BMC Cancer. 2012.

Ullio C et al. Sphingosine mediates TNFα-induced lysosomal membrane permeabilization and ensuing programmed cell death in hepatoma cells . J Lipid Res. 2012.

De Luna-Bertos E et al. Effect of Aspirin on Cell Growth of Human MG-63 Osteosarcoma Line. ScientificWorldJournal. 2012.

Luchetti F. et al. Fas Signalling Promotes Intercellular Communication in T Cells. PLoS One. 2012.

Veliz-Rodriguez T et al. Role of Toll Interleukin-1 Receptor (IL-1R) 8, a Negative Regulator of IL-1R/Toll-Like Receptor Signaling, in Resistance to Acute Pseudomonas aeruginosa Lung Infection. Infect Immun. 2012

Aveic S et al. BAG1: The Guardian of Anti-Apoptotic Proteins in Acute Myeloid Leukemia. PLoS One. 2011.

Calvanese V. et al. A promoter DNA demethylation landscape of human hematopoietic differentiation. Nucleic Acids Res. 2012

Caraballo JM et al. High p27 protein levels in chronic lymphocytic leukemia are associated to low Myc and Skp2 expression, confer resistance to apoptosis and antagonize Myc effects on cell cycle. Oncotarget. 2014.

Sakkas A. et al. Safety and Efficacy of Suicide Gene Therapy with Adenosine Deaminase 5-Fluorocytosine Silmutaneously in in Vitro Cultures of Melanoma and Retinal Cell Lines. J Cancer. 2014.

Fernández-Sánchez A. et al. DNA demethylation and histone H3K9 acetylation determine the active transcription of the NKG2D gene in human CD8+ T and NK cells. Epigenetics. 2013

Bonvini P. et al. The effect of the cyclin-dependent kinase inhibitor flavopiridol on anaplastic large cell lymphoma cells and relationship with NPM-ALK kinase expression and activity. Haematologica. 2009.

Airoldi I . et a. IL-12 Can Target Human Lung Adenocarcinoma Cells and Normal Bronchial Epithelial Cells Surrounding Tumor Lesions. PLoS One. 2009.

Herrero-Martín D. et al. Stable interference of EWS–FLI1 in an Ewing sarcoma cell line impairs IGF-1/IGF-1R signalling and reveals TOPK as a new target. Br J Cancer. 2009

Fernández-Sánchez A. et al. DNA demethylation and histone H3K9 acetylation determine the active transcription of the NKG2D gene in human CD8+ T and NK cells. Epigenetics. 2013

Grazu V. et al. Application of magnetically induced hyperthermia in the model protozoan Crithidia fasciculata as a potential therapy against parasitic infections. Int J Nanomedicine. 2012

 Marcuzzi A. et al. Lovastatin induces apoptosis through the mitochondrial pathway in an undifferentiated SH-SY5Y neuroblastoma cell line. Cell Death and Disease (2013)

Panuzzo C. et al. New alternative splicing BCR/ABL-OOF shows an oncogenic role by lack of inhibition of BCR GTPase activity and an increased of persistence of Rac activation in chronic myeloid leukemia. Oncoscience. 2015

Tricarico PM. Et al. Alendronate, a double-edged sword acting in the mevalonate pathway. Mol Med Rep. 2015

Celsi F. et al. Knockdown of MVK does not lead to changes in NALP3 expression or activation. J Inflamm (Lond). 2015

Jimenez S. et al. Disruption of Amyloid Plaques Integrity Affects the Soluble Oligomers Content from Alzheimer Disease Brains. PLoS One. 2014

Ferreiro SF. et al. In vitro chronic effects on hERG channel caused by the marine biotoxin Azaspiracid-2. Toxicon. Author manuscript; available in PMC 2015

Taiana MM. Et al. Neutralization of Schwann Cell-Secreted VEGF Is Protective to In Vitro and In Vivo Experimental Diabetic Neuropathy. PLoS One. 2014

Tricarico PM. Et al. Block of the Mevalonate Pathway Triggers Oxidative and Inflammatory Molecular Mechanisms Modulated by Exogenous Isoprenoid Compounds. Int J Mol Sci. 2014

Sousa MC. Et al. Antileishmanial Activity of Semisynthetic Lupane Triterpenoids Betulin and Betulinic Acid Derivatives: Synergistic Effects with Miltefosine. PLoS One. 2014

Mezzaroba N. et al. New Potential Therapeutic Approach for the Treatment of B-Cell Malignancies Using Chlorambucil/Hydroxychloroquine-Loaded Anti-CD20 Nanoparticles. PLoS One. 2013

Tricarico PM. Et al Temperature and Drug Treatments in Mevalonate Kinase Deficiency: An Ex Vivo Study. Biomed Res Int. 2013

Zanin V et al. Lovastatin Dose-Dependently Potentiates the Pro-inflammatory Activity of Lipopolysaccharide Both In Vitro and In Vivo. J Cardiovasc Transl Res. 2013

Bartolome A. et al. Autophagy plays a protective role in endoplasmic reticulum stress-mediated pancreatic β cell death. Autophagy. 2012.

Larghi P et al. The p50 Subunit of NF-κB Orchestrates Dendritic Cell Lifespan and Activation of Adaptive Immunity. PLoS One. 2012

Suarez-Alvarez B et al. Epigenetic Mechanisms Regulate MHC and Antigen Processing Molecules in Human Embryonic and Induced Pluripotent Stem Cells. PLoS One. 2010

Ocio EM. Et al. In vitro and in vivo rationale for the triple combination of panobinostat (LBH589) and dexamethasone with either bortezomib or lenalidomide in multiple myeloma. Haematologica. 2010

Bejarano I. et al. Role of Calcium Signals on Hydrogen Peroxide-Induced Apoptosis in Human Myeloid HL-60 Cells. Int J Biomed Sci. 2009.

Stanzione R. et al. C2238/αANP modulates apolipoprotein E through Egr-1/miR199a in vascular smooth muscle cells in vitro. Cell Death Dis. 2015.

Galán-Bobo A. et al. Overexpression of AQP3 Modifies the Cell Cycle and the Proliferation Rate of Mammalian Cells in Culture. PLoS One. 2015

Lopez-Cacho JM et al. Association of Immunological Cell Profiles with Specific Clinical Phenotypes of Scleroderma Disease. Biomed Res Int. 2014

Mendes J. et al. L744,832 and Everolimus Induce Cytotoxic and Cytostatic Effects in Non-Hodgkin Lymphoma Cells. Pathol. Oncology.

Muzio M. et al. Expression and function of toll like receptors in chronic lymphocytic leukaemia cells. British Journal of Haematology. 2009,

Sanchez-Paradinas S. et al. Enhanced cytotoxic activity of bile acid cisplatin derivatives by conjugation with gold nanoparticles. Journal of Inorganic Biochemistry. 2014.

Misiewicz-Krzeminska I et al. Restoration of microRNA-214 expression reduces growth of myeloma cells through positive regulation of P53 and inhibition of DNA replication. Haematologica. 2013.

Gabas IM. Et al. In vitro cell cytotoxicity profile and morphological response to polyoxometalate-stabilised gold nanoparticles.  New J. Chem., 2016.

Taberna F. et al. Biological quality control for extracorporeal photochemotherapy: Assessing mononuclear cell apoptosis levels in ECP bags of chronic GvHD patients. 2015 Journal of Clinical Apheresis.

Liberal J. et al. Chemical characterization and cytotoxic potential of an ellagitannin-enriched fraction from Fragaria vesca leaves. 2015 Arabian Journal of Chemistry.

Luchetti F. et al. 7-Ketocholesterol and 5,6-secosterol induce human endothelial cell dysfunction by differential mechanisms. 2015 Steroids.

  • Annexin A5 
  • 308
  • 35,9 kDa

Tech Data Sheet

TDS_ANXVKF_ES.pdf

Hoja técnica

TDS_ANXVKF_EN.pdf

Technical Data Sheet

Material safety Data Sheet

MSDS_ANXVKF_EN_IVD.pdf

Technical Data Sheet