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Supernatant proceeding from an in vitro cell culture of a cell hybridoma
Mouse, rat and human
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
Isotype control IgG2a, clone B12/8, is mAb against synthetic hapten, not present in mammalian. It is effective for direct IF staining of human tissue FCM analysis using ≤1 μg/106 cells.
Today, all techniques used for both diagnosis and research must have negative controls. In Flow Cytometry, these are known as Isotypic Controls. With these it is possible to ensure that our results are reliable.The antibody specificity of the mouse IgG2a produced by clone B12/8 was chosen as an isotype control after screening on a variety of live mouse, rat, and human tissues.